Next Generation Sequencing facilitates to identify the differential expression genes of liver tissue from with liver injury(AST>40) or without liver injury(AST<40) from HBV-infected patients.. Next Generation Sequencing facilitates to identify the differential expression genes of liver tissue from with liver injury(AST>40) or without liver injury(AST<40) from HBV-infected patients.

We obtained HBV-infected liver tissues from liver surgery(AST40) to perform next-generation sequencing (NGS). Illumina mRNA-seq sample preparation kit was used to perform paired-end library sequencing with Illumina HiSeq 2000 and sequence analysis was determined using the Illumina data analysis pipeline. Based on the comparison results, we performed the GO and KEGG pathway enrichment analysis. That might suggest the different status of HBV-infected liver from liver surgery(AST40). Overall design: Differential gene expression between liver tissue (AST40).