Two distinct trophectoderm lineage stem cells from human pluripotent stem cells

We report the derivation of two distinct stem cells of the trophectoderm lineage from human pluripotent stem cells. The first is a CDX2- stem cell equivalent to primary hTSCs – they both exhibit identical expression of key markers, are maintained in culture and differentiate under similar conditions, and share high transcriptome similarity. The second is a CDX2+ putative human trophectoderm stem cell (hTESC) with distinct cell culture requirements and differences in gene expression and differentiation relative to hTSCs. RNA sequencing was conducted on the following cells/conditions: two primary hTSC lines (CT29, CT30), undifferentiated hESCs (two cell lines - H1, H9), hESCs after 3 days of differentiation to trophoblast (H1-day3, H9-day3), hTSCs derived from hESCs (H1-hTSC, H9-hTSC), hTESCs derived from hESCs (H1-hTESC, H9-hTESC). Four biological replicates were used for each cell type/condition (10x4=40 samples total)