Estimate of L. mexicana RNA in mixed RNA samples from infected BMDMs.
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Peak sizes of the LSU β and 28S rRNA subunits were measured in arbitrary fluorescence units (AFU) on an Agilent Bioanalyzer. To estimate the proportion of L. mexicana RNA in the sample, AFU were divided by length of the corresponding rRNA to determine the relative amounts of leishmanial and murine rRNAs. The number of amastigotes per 100 macrophages was determined by light microscopy.Estimate of L. mexicana RNA in mixed RNA samples from infected BMDMs.
创建时间：
2015-12-03
