HOMER_peak_files.tar

Using HOMER with default settings, peaks for histone marks and Flag tagged Hairy protein were identified using signals from H3 ChIP and input respectively as background. Peaks called by HOMER were associated with genes by identifying the nearest RefSeq TSS and annotated to a genomic feature such as intergenic, intron, exon etc.本研究采用默认参数运行HOMER，分别以H3 ChIP与Input样本的信号作为背景，鉴定得到组蛋白修饰峰与Flag标签融合Hairy蛋白的结合峰。随后，通过匹配最近的RefSeq转录起始位点（Transcription Start Site, TSS），将HOMER鉴定出的峰与对应基因建立关联，并将其注释为基因间区、内含子、外显子等基因组特征类型。