ONT Flongle sequencing of clinical samples

In this work, we investigate whether the Oxford Nanopore Technology´s (ONT) Flongle sequencing platform is suitable for real-time sequencing directly from blood cultures to identify bacteria and detect resistance-encoding genes. Pure bacterial cultures of four clinical isolates of Escherichia coli and Klebsiella pneumoniae and two spiked blood cultures were used for the analysis. We have sequenced both the whole genome and plasmids, isolated from these bacteria using two different sequencing kits.