RNA seq for HLECs transfected with siIFITM1

To understand the role of IFITM1 in HLECs, HLECs were transfected with siIFITM1 or sicontrol . RNAs isolated from these cells were subjected to RNA seq analysis. DOI: 10.1096/fj.202500783R Overall design: HLECs were transfected with a 5 nM siRNA pool against IFITM1 (Invitrogen, Carlsbad, CA, USA) or nontarget control (Invitrogen, Carlsbad, CA, USA) as previously described for 3 days. Then, cells were processed for total RNA isolation using miRNeasy kit (Qiagen, Hilden, Germany). Expression profiling was conducted using a Next-Generation Sequencing platform at Northwestern University Center for Genetic Medicine core. Gene differential expression profiles obtained from RNA-seq analysis were subjected to gene ontology analysis using DAVID.