Quantitative histogram analysis of images

Abstract A routine for histogram analysis of images has been written in the object-oriented, graphical development environment LabVIEW. The program converts an RGB bitmap image into an intensity-linear greyscale image according to selectable conversion coefficients. This greyscale image is subsequently analysed by plots of the intensity histogram and probability distribution of brightness, and by calculation of various parameters, including average brightness, standard deviation, variance, minimal and... Title of program: HAWGC - histogram analysis with grayscale conversion Catalogue Id: ADXG_v1_0 Nature of problem Quantification of image data (e.g., for discrimination of molecular species in gels or fluorescent molecular probes in cell cultures) requires proprietary or complex software packages, which might not include the relevant statistical parameters or make the analysis of multiple images a tedious procedure for the general user. Versions of this program held in the CPC repository in Mendeley Data ADXG_v1_0; HAWGC - histogram analysis with grayscale conversion; 10.1016/j.cpc.2006.06.014 This program has been imported from the CPC Program Library held at Queen's University Belfast (1969-2019)摘要 本程序基于面向对象的图形化开发环境LabVIEW编写，用于实现图像直方图分析。程序可根据可选的转换系数，将RGB位图转换为强度线性化的灰度图像。随后，通过绘制强度直方图与亮度概率分布曲线，并计算各项统计参数（包括平均亮度、标准差、方差、最小值及……）对该灰度图像进行分析。 程序名称：HAWGC——灰度转换直方图分析（Histogram Analysis with Grayscale Conversion） 目录编号：ADXG_v1_0 问题本质 图像数据量化工作（例如用于区分凝胶中的分子种类或细胞培养中的荧光分子探针）往往需要使用专有或复杂的软件包，但这类软件要么未涵盖所需的统计参数，要么会让普通用户处理多张图像的分析流程变得繁琐冗长。 Mendeley数据平台CPC程序库收录的本程序版本 ADXG_v1_0；HAWGC——灰度转换直方图分析（Histogram Analysis with Grayscale Conversion）；10.1016/j.cpc.2006.06.014 本程序源自贝尔法斯特女王大学馆藏的CPC程序库（1969-2019年）。