Assessment of Spinal DNA Damage Following L1 Contusion Spinal Cord Injury in Female C57Bl/6 Mice
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https://odc-sci.org/data/1278
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STUDY PURPOSE: Assessing the presence, timing, and spinal location of DNA damage following spinal cord injury. DATA COLLECTED: 12-week-old female C57Bl/6 mice received a 75 kdyne contusion at spinal L1, were Sham-operated, or remained naive. At 1 hour post-injury (hpi), 1 day post-injury (dpi), 3 dpi, 7 dpi, and 28 dpi the mice were sacrificed (n = 6-10 per group).
Histological analysis of the spinal cord was performed on longitudinal sections of the spinal cord. DNA damage was labeled using gamma-H2AX, neurons by NeuN labeling, Caspase-3 mediated apoptosis by Cleaved Caspase-3 labeling, and cell nuclei using DAPI. Imaging was performed on a Zeiss Axioscan using the 647, 488, and DAPI channels.
Automatic analysis was performed using QuPath software. By thresholding, cells/neurons with gamma-H2AX foci (DNA-damaged cells), pan nuclear gamma-H2AX signal (cells with early cell death), and negative gamma-H2AX signal were identified and counted. DATA USAGE NOTES:
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Open Data Commons for Spinal Cord Injury (ODC-SCI)
创建时间：
2025-03-12
