Additional file 2: of Developmental biology and potential use of Alboglossiphonia lata (Annelida: Hirudinea) as an “Evo-Devo” model organism

Estimates of Evolutionary Divergence between Hirudinea sequences. Analyses were conducted using a Pairwise distance matrix [43]. The rate variation among sites was modeled with a gamma distribution (shape parameter = 1). The analysis involved 29 nucleotide sequences. All positions containing gaps and missing data were eliminated. There were a total of 475 positions in the final dataset. Evolutionary analyses were conducted in MEGA7 [44]. (XLSX 13 kb)