Figure 7A

A. MRC5 cells were plated on coverslips and infected with TB40/E-mCh), UL88-STOP-mCh, or UL88-Rev-mCh at MOI of 0.05. Cells were fixed day 7 and day 9 and subjected to IF analysis: DAPI (blue) and NF-KB (green), infection (red). NF-KB translocation into the nucleus was detected via staining, and cells with nuclear NF-KB signal were classified as “NF-KB positive nuclei.” Percent of NF-KB positive nuclei over total cells counted were calculated from at least 10 fields containing 100 cells each for each sample as shown in the graph. B. MRC5 cells expressing either GFP-vector or GFP-UL88 were plated on coverslips and infected with mCherry tagged WT or UL88-STOP TB40/E virus at MOI of 0.05. Cells were fixed day 7 and day 9 and subjected to IF analysis: DAPI (blue), GFP (green) and NF-KB (grey), infection (red). NF-KB translocation into the nucleus was detected via staining, and cells with nuclear NF-KB signal were classified as “NF-KB positive nuclei.” Percent of NF-KB positive nuclei over total cells counted were calculated from at least 10 fields containing 100 cells each for each sample as shown in the graph.