Whole Genome Bisulfite Sequencing (WGBS) of Alkalinity Stress and Freshwater Recovery in Amur Ide

This study examines the DNA methylation responses of tiger to alkalinity stress and subsequent recovery in freshwater. Fish were exposed to 50 mmol/L bicarbonate alkalinity, sampled at day 1 (AW1), day 5 (AW5), and day 10 (AW10) of stress, and stored at -80 degrees Celsius. A control group in freshwater for 30 days underwent identical sampling (FW). After stress, fish were transferred to freshwater for recovery, sampled on days 1 (FW1), 5 (FW5), and 10 (FW10), and stored for Whole genome bisulfite sequencing.