Colocalization of VAMP7 with organelle markers.

MDCK cells cultured on Transwell supports were transfected with VAMP7 constructs and where indicated, with tagged organelle markers or stained for endogenous organelle markers. Imaris software was used to determine the Pearson’s correlation coefficient between the markers. An R value of 1.0 would indicate perfect colocalization, however, variability in intensities of each channel can decrease the coefficient.