Occurrences of Apis mellifera filamentous virus (AmFV) sequences in public accessions of Apis mellifera and Varroa destructor

Honey bees (Apis mellifera), a critical agricultural pollinator in many areas, have a high rate of infection with a large DNA virus, Apis mellifera filamentous virus (AmFV), yet little is known about its ecology or impact on honey bee colonies, other than its ubiquity and apparent low virulence. This study scanned over 5,000 public data sets to detect AmFV sequences in honey bees as well as a parasitic mite of honey bees, Varroa destructor, that is a potential vector of AmFV. The data release consists of these files: 1. AmFV.genome.assemblies.aligned.fas, which contains new AmFV draft genome sequences generated by this study aligned with existing reference genome accessions downloaded from the National Center for Biotechnology Information (NCBI). 2. kmer.list.txt, a list of kmers that were extracted from reference sequences and searched for in Sequence Read Archive (SRA) accessions. 3. sample.metadata.txt, which lists all accessions of the SRA, and NCBI database of high-throughput sequence data, that were used in this study. The file also includes the raw occurrence counts of kmers listed in kmer.lists.txt, summed by category.西方蜜蜂（Apis mellifera）作为诸多地区至关重要的农业传粉昆虫，其感染大型DNA病毒——西方蜜蜂丝状病毒（Apis mellifera filamentous virus, AmFV）的比例极高，但目前学界对该病毒的生态学特性及其对蜂群的影响仍知之甚少，仅明确其分布广泛且致病力看似较低。本研究检索了超过5000个公开数据集，以检测蜜蜂及其寄生螨——狄斯瓦螨（Varroa destructor，该螨被认为是AmFV的潜在传播媒介）中的AmFV序列。本次公开的数据包含以下文件：1. AmFV.genome.assemblies.aligned.fas：包含本研究新生成的AmFV草图基因组序列，以及从美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）下载的现有参考基因组序列的比对结果。2. kmer.list.txt：包含从参考序列中提取的k元组（k-mer）列表，该列表将被用于在序列读序存档（Sequence Read Archive, SRA）条目中进行检索。3. sample.metadata.txt：列出了本研究所用的全部SRA及NCBI高通量测序数据库条目，该文件同时包含按类别汇总的、来自kmer.lists.txt中所列k元组的原始出现计数。