Annexin-V assay of stallion spermatozoa stored during five days (day 1 D1 to day 5 D5) at 5°C FE fresh extended sperm, CC sperm processed through colloidal centrifugation.

Live % percentage of live sperm, A+, annexin positive sperm, spermatozoa depicting translocation of PS, A+/PI dead spermatozoa depicting PS translocation, A-PI+ necrotic spermatozoa Within a row values with different superscript differ statistically a-b p<0.01. (Means ± SD) Results are derived from 28 identical experiments (7 stallions, 4 ejaculates per stallion).