Transcriptional profiling of mature murine adipocytes of the inguinal white adipose tissue depot following LPCAT3 deletion and dietary PUFA manipulation [bulk RNA-seq]. Transcriptional profiling of mature murine adipocytes of the inguinal white adipose tissue depot following LPCAT3 deletion and dietary PUFA manipulation [bulk RNA-seq]

To analyze the contribution of dietary PUFAs and adipocyte LPCAT3 expression in modulating the transcriptome of murine inguinal white adipose tissue We performed bulk RNA sequencing with a two-factor experimental design. WT and AdL3KO mice on 3 high-fat diets (60% calories from fat) - omega-6 enriched (n6), saturated-enriched (SFA), and omega-3 enriched (n3). Overall design: Comparative gene expression profiling analysis of iWAT from WT and AdL3KO mice on 3 high-fat diets (60% calories from fat) - omega-6 enriched (n6), saturated-enriched (SFA), and omega-3 enriched (n3).