Properties of arenavirus-derived HLA-A*0201-restricted CD8⁺ T cell epitopes.

aPeptide position within prototypic strains of arenavirus GPC, NP, L and Z proteins. bAverage % amino acid identity of epitope sequence to all full-length isolate sequences within a given arenavirus species. cEach peptide was screened for its ability to be endogenously processed and presented by HLA-A*0201. Purified CD8+ T cells from peptide-immunized HLA-A*0201 mice were tested for recognition of human JA2.1 cells infected with the appropriate rVACV construct expressing one of the arenavirus proteins. +, Processed; +/−, weakly processed; −, not processed. dPeptides were tested for binding to the HLA-A*0201 allele. Bold font highlights biologically relevant binders (IC50≤500 nM). −, peptides with binding affinity >5000 nM.