Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes

Raw multichannel and/or Z-stack source data from time series images in TIF format to accompany publication of: <strong>Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes</strong> Jina Ko¹, Martin Wilkovitsch², Juhyun Oh¹, Rainer Kohler¹, Evangelia Bolli^1,3, Mikael J. Pittet^1,3,4,5, Claudio Vinegoni¹, David B. Sykes^6,7, Hannes Mikula², Ralph Weissleder^1,8*, Jonathan C. T. Carlson^1,7* ¹Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114 ² Institute of Applied Synthetic Chemistry, TU Wien, 1060 Vienna, Austria ³ Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland ⁴ Ludwig Institute for Cancer Research, Lausanne Branch, Switzerland ⁵ AGORA Cancer Center, Lausanne, Switzerland ⁶ Center for Regenerative Medicine, Massachusetts General Hospital, Boston, MA, USA ⁷Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA ⁸Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115