Infectious bursal disease virus Genome sequencing and assembly. Infectious bursal disease virus

In order to develop a method for the purification of IBDV using Immobilized Metal-ion Affinity Chromatography (IMAC), a project is proposed to generate the wild-type of recombinant P3009 IBDV and the P3009 IBDV mutants with site-directed mutagenesis at P domain (residue 206-350) of VP2 by reverse genetics. This project not only aims to establish and efficient process for IBDV vaccine production and purification to prevent IBD, but also to establish a novel strategy for the production and purification of other viruses or virus-like particles.