16S rRNA gene (from RNA) from samples collected on cruise Chikyu-331 in the Okinawa Trough, Japan from September to October 2010

<p>Next-generation sequencing data across a hydrothermal gradient; Okinawa Backarc Basin IODP 331 – subvent biosphere.&nbsp;RNA extractions were attempted from sediments at Sites C0014 and C0017 to selectively investigate the active microbial populations at these sites. This study was conducted to supplement the gene-based and metagenomic studies. However, the results from sequenced reverse transcribe 16S rRNA demonstrate the sensitivity of extraction and amplification to external nucleic acids and attest to the challenges in working with low biomass environmental samples. Inconsistencies between primersets and replicates make conclusions about the results of this RNA study extremely tenuous.</p>热液梯度下的下一代测序（Next-generation sequencing）数据；冲绳弧后盆地IODP 331航次——俯冲带下方生物圈。本研究尝试从C0014与C0017站位的沉积物中提取RNA，以选择性探究两处站位的活性微生物种群。本研究旨在补充基于基因的宏基因组学（Metagenomics）相关研究。然而，经测序的反转录16S rRNA（Reverse-transcribed 16S rRNA）结果显示，提取与扩增过程对外源核酸具有敏感性，同时也证实了处理低生物量环境样本时所面临的诸多挑战。引物组与重复样本间存在的不一致性，使得本RNA研究的结论极为站不住脚。