Evaluating bread wheat varietal responses to late-spring frost conditions

This study investigates the impact of late-spring Frost (LSF) stress on wheat (Triticum aestivum) during critical reproductive stages. A factorial experiment was conducted with three wheat genotypes ('Roshan', 'Falat', and 'Superhead'), three growth stages (pollen development, spike emergence, and pollination), and three stress levels (normal, one-day stress, and four-day stress) in a completely randomized design with nine replications. Analysis of variance and mean comparison (LSD method) showed that wheat varieties responded differently to growth stages and stress durations, with 'Roshan' being more sensitive and 'Superhead' more adaptable. Proline accumulation and increased photosynthetic pigment levels were identified as stress resistance mechanisms, though they did not fully prevent yield reductions. Principal Component Analysis (PCA) explained 75.42% of the variation, showing negative correlations between yield-related traits (grain number and grain weight) and the first component..., , # Data on the Impact of Late-Spring Frost Stress on Wheat Reproductive Traits

\[Access this dataset on Dryad](Dataset DOI link)

This dataset originates from a factorial experiment assessing the impact of late-spring frost (LSF) stress on reproductive traits in wheat (*Triticum aestivum*). The experiment investigated physiological and yield-related responses in three wheat genotypes—'Roshan', 'Falat', and 'Superhead'—when subjected to LSF during three critical growth stages: pollen development, spike emergence, and pollination. Frost stress was applied at two intensities: one-day LSF and four-day LSF, alongside a no-stress control group. A completely randomized design (CRD) was used with nine replicates per treatment. The resulting data provide insights into how reproductive-stage frost affects key physiological traits and yield components.

## Description of the data and file structure

The dataset comprises mean values calculated from nine replicates for each treatment group (genotyp...,