aAcetylation was confirmed by MS and MS/MS of the peptide. bNumbering according Genbank accession # P14136; underline indicates the acetylated lysine. cMonoisotopic mass of the neutral peptide. dMasco
In order to identificate NLRP3 acetylation, we utilized an acetylproteomic approach to identify the acetylation sites within NLRP3. We first reconstituted the NLRP3 inflammasome in HEK293T cells by co