RNA Sequencing of human THP-1 derived macrophages transcriptomes

THP-1 cells were treated with phorbol 12-myristate 13-acetate (PMA) at 25µg/ml for 48h.Then remove PMA and rest for 24h to obtain M0 macropahges. Further applied LPS and IFN? or IL-4 and IL-13 for 24h to obtain M1, M2 macrophages, respectively. During polarization, cells were growth within control medium(LN) or medium with extra lipid mixture(LH).M0,M1,M2 cells were collected and applied to RNA-sequencing. We found that differential activation of macrophage had distinct metabolic signature.Extra lipids supplement did not significantly alter transcriptomes of macrophages. Overall design: mRNA profiles of 3 different activation states of THP-1 derived macrophages within growth medium with/without extra lipid mixture were examined by Illumina Hiseq.