Additional file 4 of Dimethyl fumarate reduces TNF and Plasmodium falciparum induced brain endothelium activation in vitro

Additional file 4. Differentially expressed pathways in DMF pretreated TNF treated HBMVEC compared to TNF treatment. HBMVEC were preincubated with DMF (50 μM) for 1 h prior to the addition of TNF incubation (10 ng/ml) or TNF incubation alone for 6 h. Total RNA was extracted, reverse-transcribed, amplified and hybridized to a microarray. Gene expression profiles were generated using a robust multi-array average algorithm followed by quantile normalization and batch correction. Data represents three independent experiments, each done in triplicate. 76 Ingenuity Canonical Pathways were significantly differentially expressed with p value calculated by a right tailed Fisher’s exact test. (p < 0.01, Z score at least 2 SD above the mean).