Single-cell RNA-seq in a comparative primate skeletal cell culture model [2]

We evaluated the degree to which gene expression patterns in primate skeletal cells differ across cell types and species. First, we optimized differentiation protocols of human and chimpanzee iPSCs through an intermediate MSC state and into osteogenic cells. Single-cell RNA sequencing was collected from each stage of differentiation (iPSCs, MSCs, and osteogenic cells) using the 10X Genomics platform to characterize gene expression variation. Six human iPSC lines and six chimpanzee iPSC lines were differentiated into MSCs and osteogenic cells.