Specific activity^* (U/mg) of LipT and LipS on <i>p</i>NP esters.

CalB (purchased from Sigma-Aldrich, Buchs, Switzerland) and ROL (purchased from Fluka/Sigma-Aldrich, Buchs, Switzerland) were used as references. The extinction was measured spectrophotometrically against an enzyme-free blank with 1)0.33 mM substrate solution (final concentration in 0.05 M Soerensen buffer pH 8.0 containing 0.1% gum arabic, 5 mM sodium deoxycholate and 10% DMSO) after incubation at 60°C (CalB) or 65°C (LipT, LipS, ROL) at 410 nm (ε = 7,392 M−1 cm−1). 2)0.5 mM substrate solution (final concentration in 0.1 M PB pH 8.0) after incubation at 70°C at 405 nm (ε = 19,454 M−1 cm−1). *Specific activity: n. d., not determined; −, no detectable activity or <0.01 U/mg; +, 0.01–0.30 U/mg; + +, 0.31–0.60 U/mg; + + +, 0.61–0.90 U/mg; + + + +, 0.91–1.26 U/mg. Specific activities of CalB and ROL refer to the dry-weight of the lyophilisate. Data are mean values of three independent measurements.