Quantitative proteomic profiling of the human ovary from early to mid-gestation reveals protein expression dynamics of oogenesis and folliculogenesis.

To understand the protein pathways important to human ovarian development, we performed a quantitative mass spectrometry analysis of human fetal ovarian tissue from 47, 108, 122, and 137 days of development as well as in healthy adult ovarian cortex tissue. By assessing these stages of development we were able to identify proteins that varied in expression from stages of ovarian development spanning primordial germ cell proliferation (at day 47) to oogonial differentiation and follicle formation (at day 137). We identified 3,837 proteins spanning these developmental stages (Table S1) and performed an SOM clustering analysis to identify proteins that were up-regulated between 47-137 days of developmental in comparison to adult tissue (Table S2). We also identified proteins that were differentially expressed between 47 and 137 days of development (Table S3) to identify pathways important in follicle development.

为阐明调控人类卵巢发育的关键蛋白质通路，我们对发育天数分别为47、108、122及137天的人类胎儿卵巢组织，以及健康成人卵巢皮质组织开展了定量质谱分析（quantitative mass spectrometry analysis）。通过对上述发育阶段进行系统解析，我们成功鉴定出覆盖人类卵巢发育全周期（从第47天的原始生殖细胞增殖阶段，至第137天的卵原细胞分化与卵泡形成阶段）的差异表达蛋白质。本研究共鉴定出覆盖上述发育阶段的3837种蛋白质（补充表S1），并开展了SOM聚类分析（Self-Organizing Map clustering analysis），以筛选得到相较于成人组织，在发育第47至137天期间表达上调的蛋白质（补充表S2）。此外，我们还鉴定出发育第47天与第137天之间存在差异表达的蛋白质（补充表S3），以此明确卵泡发育过程中的关键调控通路。