Steps of the High-Throughput PI5P4Kα Assay.
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https://figshare.com/articles/dataset/_Steps_of_the_High_Throughput_PI5P4K_945_Assay_/181081
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Step Notes.
1. Dispensed into white solid-bottom 1536-well MB Greiner plates with a Flying Reagent Dispenser (FRD). Reagent is kept on ice. Final concentration is 10 nM PI5P4Kα, 75 µM PI5P and 150 µM DPPS.
2. Plates centrifuged at 300×g for 10 seconds.
3. DMSO compound solutions transferred with a Kalypsys pintool.
4. Dispensed with a FRD. Final concentration is 5 µM ATP.
5. Plates incubated at room temperature.
6. Dispensed with a FRD.
7. Plates incubated at room temperature.
8. Dispensed with a FRD.
9. Plates incubated at room temperature.
10. Luminescence was measured with a ViewLux CCD Imager with a 20 second exposure time.
创建时间：
2013-01-10
