Data and code for mapping human nutrient-overload programs onto cell states across MASLD progression

This dataset contains the study-generated derived data, frozen analytical resources, publication source data, quality-control outputs, audit materials, and versioned R code supporting an integrative public-data study of human nutrient-overload programs across metabolic dysfunction-associated steatotic liver disease (MASLD). The core analytical framework links four public omics resources with distinct evidentiary roles. GSE200418 was used as a direct human precision-cut liver slice perturbation anchor for deriving nutrient-response programs. GSE202379 provided donor-aware single-nucleus RNA-sequencing data for mapping these frozen programs across human MASLD cell types and cross-sectional disease states, with donors rather than nuclei or tissue specimens treated as the independent biological units. PXD051911 provided hierarchical same-study multicompartment human proteomic support. GSE312698 provided a bounded, panel-restricted, FOV/reconstructed-specimen-level descriptive spatial projection in human liver CosMx data. The release includes ranked programs and directional gene sets, identifier harmonization resources, donor-aware cross-layer results, proteomic evidence summaries, bounded spatial-projection outputs, publication figures and tables with source data, reproducibility and quality-control records, and the retained analysis code chain. Primary repository data are not redistributed. They should be obtained directly from NCBI GEO and ProteomeXchange/PRIDE under accession numbers GSE200418, GSE202379, GSE312698, and PXD051911. Controlled-access materials are not included. The data support cross-dataset correspondence and orthogonal consistency analyses. They should not be interpreted as evidence of longitudinal disease progression, dietary causality, therapeutic efficacy, or an optimal intervention window.本数据集包含本研究生成的衍生数据、固化分析资源、已发表文献源数据、质量控制产出、审核材料以及带版本标识的R代码，用于支撑一项针对人类代谢功能障碍相关脂肪性肝病（metabolic dysfunction-associated steatotic liver disease, MASLD）患者营养过载程序的整合公共数据研究。 本研究的核心分析框架关联了四类具备不同证据作用的公共组学资源。GSE200418被用作人类精准切割肝切片扰动锚点，用于推导营养响应程序；GSE202379提供供体感知型单核RNA测序数据，用于在人类MASLD细胞类型及横断面疾病状态中映射上述固化程序，且以供体而非细胞核或组织标本作为独立生物学单位；PXD051911提供了同研究层级化多分区人类蛋白质组学支撑数据；GSE312698提供了人类肝脏CosMx数据中受范围限制、经面板限定的视野（field of view, FOV）/重建标本级描述性空间投影数据。 本次发布的数据集包含排序后的分析程序及定向基因集、标识符整合资源、供体感知型跨层分析结果、蛋白质组学证据摘要、受范围限制的空间投影产出、附带源数据的正式发表用图表、可重复性与质量控制记录，以及留存的完整分析代码链。 原始存储库数据未进行重新分发，用户需通过国家生物技术信息中心基因表达综合数据库（NCBI GEO）及ProteomeXchange/PRIDE数据库，以登录号GSE200418、GSE202379、GSE312698及PXD051911直接获取。本数据集未包含受控访问相关材料。 本数据集可用于开展跨数据集对应性分析及正交一致性验证分析，不得将其解读为纵向疾病进展、饮食因果关系、治疗效果或最佳干预窗口的相关证据。