Proteomics of an artificial mixture of three microorganisms to evaluate sample preparation methods for tandem mass spectrometry proteotyping

This project provides tandem mass spectrometry datasets of an artificial mixture used to optimize the lysis and evaluate digestion protocols for a fast preparation of peptides for tandem mass spectrometry proteotyping. The microorganism mixture is composed of Bacillus subtilis subsp. spizizenii ATCC 6633, Escherichia coli BL21-Gold(DE3)pLysS AG and Saccharomyces cerevisiae S288C.