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Arabidopsis meiotic-specific recombinase DMC1 IP-MS

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To search for regulators of DMC1 stability, we generated DMC1-FLAG transgenic Arabidopsis plants and conducted immunoprecipitation-mass spectrometry (IP-MS) assay. 6,477 peptides corresponding to 2309 proteins were identified and 1009 proteins remained after removing background interactions using plants transformed with the corresponding empty vector (FLAG). GO term analysis was performed with the obtained data and revealed that ubiquitin proteasome pathway components are significantly enriched among the candidate proteins.
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2023-10-24
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