Gene Expression Changes in Soleus Muscle in Mice after Pericytes-Deletion

Pericytes(PCs) are mural cells embedded in the capillary basal lamina. Some PC populations exhibit multipotency, similar to mesenchymal stem cells. Here, the the process of adaptation was investigated by determining changes in transcript profiles when NG2 positive PCs were deleted. PC-deletion was induced by using NG2CreER(+/-)/diphtheria toxin flagment A(DTA) transgenic mice. Cre recombinase was induced by tamoxifen(Tam) treatment(intraperitoneally, 100mg/kg body weight per day) for 5days. Samples were collected at 1month after Tam treatment. Analysis used RNA of NG2Cre(-/-)/DTA mice with Tam treat as control sample for comparison to the experimental samples. Each RNA was extracted from four soleus muscles of two mice.