Genome-wide analysis of RNA Pol II CTD modifications in resonse to lysine deacetylase inhibition

To investigate the role of RNA Pol II CTD acetylation we performed comprehensive deacetylase inhibition using common small-molecule inhibitors and performed ChIP-seq from mouse cells using antibodies against RNA Polymerase II CTD modifications: (8WG16, unmodified heptads; K7ac, acetylated heptads; 4H8, S5-phosphorylated heptads). In addition, we tested the effect of knockdown of RPRD1B, a key transcription regulator that recruits RPAP2, a CTD S5-phosphatase. Examination of RNA Pol II CTD modifications in NIH3T3 cells in response to lysine-deacetylase inhibition across multiple biological replicates and conditions.