Staphylococcus aureus's Oxford Nanopore Sequencing discovers Non Contiguous Operons

Non-contiguous operons are transcriptional units consisting of a group of genes that are transcribed together despite being separated by a gene or genes that are transcribed in the opposite direction. Conventional RNA sequencing tools (such as Illumina) are not the best ones to study this specific architecture due to its limitation in transcripts length. With ONT technology, we are able to see full-length transcripts which englobe whole non contiguous operons, proving their existence.