Transcriptomic analysis of resiniferatoxin-treated urinary bladder trigone in a porcine model

Resiniferatoxin (RTX) is a potent capsaicin analogue used as a drug for experimental therapy of a range of neurogenic disorders associated with enhanced nociceptive transmission, including lower urinary tract symptoms. The present study investigated the transcriptomic analysis of the porcine urinary bladder trigone after intravesical RTX instillation. The trigone is an area located between two ureteral orifices and the internal urethral meatus. The analysis was performed on 12 juvenile female pigs. Six pigs served as controls and were treated with intravesical instillation of a 5% aqueous solution of ethyl alcohol. Another group of 6 pigs was treated with RTX by intravesical instillation of the toxin. One week after the administration of the aqueous solution of ethyl alcohol or RTX, a midline laparotomy was performed in all the animals, and samples of the urinary bladder trigone were cut out for transcriptome analysis. Within both the experimental and control groups total RNA was isolated and high-throughput RNA sequencing was used to analyze the global changes in the transcriptome of the collected parts of the urinary bladder trigones after RTX treatment.