HIV Env Nanodisc B cell sorting

SA684 mice were immunized with mRNA-LNPs encoding Env gp151 MPER ND and memory (CD19+IgM-IgD-) and naïve (CD19+IgM+IgD+) B cells were analyzed by flow cytometry. Antigen-specific (Env gp151 MPER Nanodisc++) and epitope-specific (KO-) B cells were detected using immunogen-matched WT and 10E8-epitope KO nanodisc probes and WT nanodisc++ cells were sorted and their BCRs sequenced.