Statistical analyses of the results in MRE prediction before and after filtering with the central loop score

TP: true positive; FP: false positive; FN: false negative; TN: true negative.
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1.Old criteria: seed homology plus minimum free energy <−15 kcal/mol, not filtered by the central loop score. Detailed description can be found in the Methods section.
2.New criteria: seed homology plus free energy <−15 kcal/mol, filtered by the central loop score.
3.Gold Standard: Luciferase activity assay.
4.**: The specificity comparison between old criteria and new criteria shows a significant difference (p<0.01, statistics analysis using McNemar's Test).