Statistical analyses of the results in MRE prediction before and after filtering with the central loop score

TP: true positive; FP: false positive; FN: false negative; TN: true negative. ; 1.Old criteria: seed homology plus minimum free energy <−15 kcal/mol, not filtered by the central loop score. Detailed description can be found in the Methods section. 2.New criteria: seed homology plus free energy <−15 kcal/mol, filtered by the central loop score. 3.Gold Standard: Luciferase activity assay. 4.**: The specificity comparison between old criteria and new criteria shows a significant difference (p<0.01, statistics analysis using McNemar's Test).