Screening of GLP-1r agonists from natural products using affinity ultrafiltration screening coupled with UPLC-ESI-Orbitrap-MS technology: a case study of <i>Panax ginseng</i>

In our study, a method based on affinity ultrafiltration screening coupled with UPLC-ESI-Orbitrap-MS technology was established to select Glucagon-like peptide-1 receptor (GLP-1R) agonists from natural products, and as an example, the GLP-1R agonists from <i>Panax ginseng</i> was selected using our established method. As a result, total five GLP-1R agonists were selected from <i>Panax ginseng</i> for the first time. Our results indicated that activating GLP-1R to promote insulin secretion probably was another important hypoglycemia mechanism for ginsenosides in <i>Panax ginseng</i>, which had great influence on the study of the anti-diabetes effect of ginsenosides.