Spatial cartography of human thymus enables the geopositioning of lineage transcription factors in rare mimetic thymic epithelial cells

In this study, we have applied the Stereo-seq spatial transcriptomics method to establish a high-resolution spatial atlas for the human fetal thymus. We profiled fetal thymic tissues in the second trimester, comprising ages 13, 14, 17 and 18 weeks post-conception.