Data from: Immunoglobulin detection in wild birds: effectiveness of three secondary anti-avian IgY antibodies in direct ELISAs in 41 avian species

1.Immunological reagents for wild, non-model species are limited or often non-existent for many species. 2. In this study, we compare the reactivity of a new anti-passerine IgY secondary antibody with existing secondary antibodies developed for use with birds. Samples from 41 species from the following six avian orders were analysed: Anseriformes (1 family, 1 species), Columbiformes (1 family, 2 species), Galliformes (1 family, 1 species), Passeriformes (16 families, 34 species), Piciformes (1 family, 2 species) and Suliformes (1 family, 1 species). Direct ELISAs were performed to detect total IgY using goat anti-passerine IgY, goat anti-chicken IgY or goat anti-bird IgY secondary antibodies. 3.The anti-passerine antibody exhibited significantly higher IgY reactivity compared to the anti-chicken and/or anti-bird antibodies in 80% of the passerine families tested. Birds in the order Piciformes (woodpeckers) and order Suliformes (cormorants) were poorly detected by all three secondary antibodies. A comparison of serum and plasma IgY levels was made within the same individuals for two passerine species (house finch and white-crowned sparrow), and serum exhibited significantly more IgY than the plasma for all three secondary antibodies. This result indicates that serum may be preferred to plasma when measuring total antibody levels in blood. 4.This study indicates that the anti-passerine IgY secondary antibody can effectively be used in immunological assays to detect passerine IgY for species in most passerine families and is preferred over anti-chicken and anti-bird secondary antibodies for the majority of passerine species. This anti-passerine antibody will allow for more accurate detection and quantification of IgY in more wild bird species than was possible with previously available secondary antibodies.针对野生非模式物种的免疫试剂，在诸多物种中普遍存在供应不足甚至完全缺失的问题。本研究中，我们将一款新型抗雀形目免疫球蛋白Y（IgY）二抗与现有的鸟类专用二抗的反应活性进行了对比分析。本次研究共分析了隶属于以下6个鸟目的41个物种的样本：雁形目（1科1种）、鸽形目（1科2种）、鸡形目（1科1种）、雀形目（16科34种）、鴷形目（1科2种）以及鲣鸟目（1科1种）。实验采用山羊抗雀形目IgY、山羊抗鸡IgY或山羊抗鸟类IgY二抗，通过直接酶联免疫吸附试验（ELISA）检测总IgY水平。在受试的80%的雀形目科中，该抗雀形目抗体所展现出的IgY反应活性显著优于抗鸡IgY及/或抗鸟类IgY抗体。鴷形目（啄木鸟类群）与鲣鸟目（鸬鹚类群）的鸟类样本，则无法被三款二抗有效检出。本研究还针对家朱雀、白冠带鹀这两种雀形目物种的同一受试个体，对比了血清与血浆中的IgY水平，结果显示，在三款二抗的检测体系中，血清内的IgY含量均显著高于血浆。该结果表明，在检测血液总抗体水平时，血清相较于血浆更为适用。本研究证实，这款抗雀形目IgY二抗可有效应用于免疫检测实验，用于检测绝大多数雀形目科属物种的IgY；且对于多数雀形目物种而言，其性能优于抗鸡IgY与抗鸟类IgY二抗。相较于此前可用的二抗，这款抗雀形目抗体能够在更多野生鸟类物种中实现更为精准的IgY检测与定量分析。