The phenotype of ccdc80-l1-MO-injected embryos is dose-dependent.

The percentage of embryos displaying axonal defects decreased from 84% to 64% when a lower dose of morpholino was used. Both ventral and dorsal axonal pathfinding resulted impaired in the 69% of affected embryos when 12 ng/embryo of morpholino were used. After the injection of the lower dose of ccdc80-l1-MO (8 ng/embryo), 27% of affected embryos showed alteration of both ventral and dorsal axons, whereas the 37% displayed only ventral defective axons and dorsal axons alone were never affected.