CIL:24045

Starved Dictyostelium cells lacking cGMP-binding protein C (gbpC-; no guanylyl cyclase activity) and in medium supplemented with 60 µM LY294002 (a PI3K inhibitor) and 1µM cAMP migrate toward the anode (right). A direct-current electric field of 10 Vpcm was applied and the cells were observed for 20 min at 5-sec intervals. (Scale bar, 50 µm.) In contrast, cathode-directed migration is strongly attenuated in wild-type cells in the same medium. Compare with wild-type control video (CIL# 24043) and other mutants lacking guanylyl cyclase activity (CIL#24044 and 24046). Videos are supporting information in Proc Natl Acad Sci (2009). 106: 6667-6672.