Data from: Genotyping of Coxiella burnetii from domestic ruminants and human in Hungary: indication of various genotypes

Background: Information about the genotypic characteristic of Coxiella burnetii from Hungary is lacking. The aim of this study is to describe the genetic diversity of C. burnetii in Hungary and compare genotypes with those found elsewhere. A total of 12 samples: (cattle, n = 6, sheep, n = 5 and human, n = 1) collected from across Hungary were studied by a 10-loci multispacer sequence typing (MST) and 6-loci multiple-locus variable-number of tandem repeat analysis (MLVA). Phylogenetic relationships among MST genotypes show how these Hungarian samples are related to others collected around the world. Results: Three MST genotypes were identified: sequence type (ST) 20 has also been identified in ruminants from other European countries and the USA, ST28 was previously identified in Kazakhstan, and the proposed ST37 is novel. All MST genotypes yielded different MLVA genotypes and three different MLVA genotypes were identified within ST20 samples alone. Two novel MLVA types 0-9-5-5-6-2 (AG) and 0-8-4-5-6-2 (AF) (Ms23-Ms24-Ms27-Ms28-Ms33-Ms34) were defined in the ovine materials correlated with ST28 and ST37. Samples from different parts of the phylogenetic tree were associated with different hosts, suggesting host-specific adaptations. Conclusions: Even with the limited number of samples analysed, this study revealed high genetic diversity among C. burnetii in Hungary. Understanding the background genetic diversity will be essential in identifying and controlling outbreaks.研究背景：目前关于匈牙利境内贝氏柯克斯体（Coxiella burnetii）的基因型特征相关数据仍较为匮乏。本研究旨在阐明匈牙利境内贝氏柯克斯体的遗传多样性，并将其基因型与全球其他地区分离得到的基因型进行比对。本研究共纳入匈牙利全境采集的12份样本（牛源6份、羊源5份、人源1份），采用10位点间隔区序列分型（multispacer sequence typing, MST）与6位点多位点可变数目串联重复序列分析（multiple-locus variable-number of tandem repeat analysis, MLVA）进行检测分析。基于MST基因型构建的系统发育关系可展示匈牙利样本与全球其他地区采集样本间的亲缘关联。 结果：本研究共鉴定出3种MST基因型：序列型（sequence type, ST）20在其他欧洲国家及美国的反刍动物中已有报道，序列型28此前在哈萨克斯坦被鉴定，而新提议的序列型37则为新型基因型。所有MST基因型均对应独特的MLVA基因型，且仅在ST20样本中就鉴定出3种不同的MLVA基因型。在与ST28和ST37相关的羊源样本中，鉴定出2种新型MLVA型别：0-9-5-5-6-2（AG）与0-8-4-5-6-2（AF）（对应检测位点为Ms23-Ms24-Ms27-Ms28-Ms33-Ms34）。系统发育树不同分支的样本对应不同宿主，提示该病原体存在宿主特异性适应性。 结论：尽管本研究分析的样本量有限，但仍揭示出匈牙利境内贝氏柯克斯体存在较高的遗传多样性。明晰其本底遗传多样性，对于疫情的识别与防控至关重要。