16S and ITS amplicons from a native revegetation carbon project

Total genomic DNA extraction was performed using the Qiagen DNeasy PowerSoil Pro extraction kit. PCR amplification of ITS region of the fungal rRNA cluster was performed with primersprimer ITS1F specific for higher fungi (CTTGGTCATTTAGAGGAAGTAA, (Gardes and Bruns, 1993)) and the general primer ITS2 (GCTGCGTTCTTCATCGATGC, (White et al., 1990)). For bacteria, the V3-V4 region of the 16S rRNA gene was sequenced using 341F and 806R primers (CCTAYGGGRBGCASAG; GGACTACNNGGGTATCTAAT (Herlemann et al., 2011; Wasimuddin et al., 2020)). Amplicon cleaning, library preparation and subsequent sequencing was conducted at AGRF (Australia), using Illumina Sequencing MiSeq technology (paired-end reads). Data was processed across two sequencing runs.