five

RNA sequencing of shoot apical meristem in Chinese cabbage

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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA746103
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When the mutant ebm3 reached the critical point of bolting, total RNA was extracted from the SAM of mutant and wild-type line FT, with three biological replications. RNA quantity and purity was analyzed using a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit. Following purification and fragmentation, the cleaved RNA fragments were reverse-transcribed to create cDNA libraries using a mRNASeqsample preparation kit. The libraries were paired-end sequenced using an Illumina HiSeq 4000 platform.
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2021-07-13
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