Direct RNA nanopore sequencing data of total RNA from human K562 cells with sequin spike-in controls

Direct RNA sequencing data for human cell line k562 with ~10% sequins, a spike-in reference standard. <br>Generated on 22 November 2017 using 500ng total RNA extracted from k562 cells and 50ng of RNA sequins Mix A (final concentration after sample preparation = 1.51 ng/uL). Nanopore MinION flowcell FAH35825 (R9.4 chemistry), displayed 1323 active channels at commencement of run. Sequencing protocol RNA001. Base called with Albacore version 2.1.3.Includes albacore summary statistics (bundled in .tgz) and a generic quality report of the run (.pdf). <br>Sequin reference sequences and expected abundances for Mix A can be found at http://www.sequin.xyz/downloads/<br>=====<b><br></b><b>Update (7 July 2019):</b> Added fastq and sequencing summary resulting from updated base calling using guppy high-accuracy (k562_guppyHAC.tgz, guppy v3.1.5 using R9.4.1 70bps RNA pore model). <br>=====<br><b>Update (15 July 2019): </b>Raw sequencing data (.fast5) uploaded to ENA (accession PRJEB33485). Available at the following link: https://www.ebi.ac.uk/ena/data/view/PRJEB33485<br>