An epigenetic map of malaria parasite development from host to vector

Chromatin state has not been investigated in many other Plasmodium species, strains or life cycle stages, yet. Here, we used ChIP-seq to profile heterochromantin and euchromatin occupancy in P. berghei parasites as they progress through the life cycle. Additionally, we performed RNAseq of the same stages in triplicate to complete the dataset. Collectively, the generated heterochromatin, euchromatin and transcription profiles provide a detailed catalogue of epigenetic regulation in the P. berghei parasite as it progresses through the life cycle. In total, we sequenced 12 ChIP-seq samples (4 HP1-ChIP-seq samples, 4 H3K9ac-ChIP-seq samples, 4 inputs) generated from chromatin from three different Plasmodium berghei lines (2.33, 820 and 507), and four different stages (asexual blood stage, female and male gametocyte and ookinete) and we sequenced 12 RNA-seq samples of the same stages from the same lines in triplicates (3 asexual blood stages, 3 female gametocytes, 3 male gametocytes, 3 ookinetes) and used gDNA as a control for the RNA-seq protocol (mixed asexual blood stages, line 2.33).