Transcriptomic analysis of RNases knockdown in A549 cells.

The function of biomolecules is essential for maintaining homeostasis. The RNA and RNase regulatory system in maintaining cellular homeostasis is not clear. To investigate the effects of RNase on gene expression and biological functions, we respectively knocked down Ago2, ANG, DICER, DROSHA, RNase L, and RNase T2 in A549 cells and performed RNA sequencing. RNase L, ANG, Dicer, Drosha, AGO2, and RNase T2 were knocked down by siRNA transfection in A549 cells, respectively. These cells were harvested 48h after siRNA transfection, and total RNA was extracted for RNA sequencing analyses.