ScRNA-seq of murine kidneys for IR induced renal injury and fibrosis

Renal ischemia-reperfusion injury (IRI) is a common clinical event that occurs in scenarios such as kidney transplantation, cardiac surgery, or shock, which can lead to renal damage. However, the specific details of renal injury and fibrosis at different time points after ischemia-reperfusion (IR), as well as the precise mechanisms underlying the transition from acute kidney injury (AKI) to chronic kidney disease (CKD), remain to be fully elucidated. Using single-cell RNA sequencing (scRNA-seq), we isolated approximately 100,000 cells from mouse kidneys and generated a large-scale transcriptomic dataset corresponding to time points of 4 hours, 12 hours, 1 day, 5 days, 14 days, and 28 days after IR. Our preliminary analysis identified 16 distinct cell types, providing a robust database to study the changes and evolution of renal IRI from early to late stages. Overall, through repeated use of the dataset for data mining and extensive analysis, our data contribute valuable transcriptomic resources for further research into renal injury and fibrosis.