CIL:15713, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

The contractile vacuole and cortex of Didinium nasutum showing an extrusive organelle (cyrtocyst) of unknown function. Further details available at Wessenberg, H. and Antipa, G. 1968. Studies on Didinium nasutum. I. Structure and ultrastructure. Protistologica 4:427-447. TEM taken in 1966 by G. Antipa with JEM T6S operating at 50kV. Neg. 6,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 90nm thickness.纳氏叠鞘滴虫（Didinium nasutum）的伸缩泡（contractile vacuole）与表膜（cortex）区域，可见一个功能未知的挤出细胞器（extrusive organelle）——弯形囊泡（cyrtocyst）。详细信息可参见Wessenberg H与Antipa G于1968年发表的《纳氏叠鞘滴虫研究 Ⅰ：结构与超微结构》，刊载于《Protistologica》第4卷，第427-447页。该透射电子显微镜（Transmission Electron Microscope, TEM）图像由G. Antipa于1966年拍摄，使用工作电压为50kV的JEM T6S型电镜，放大倍数为6000倍。原始胶片采用爱普生（Epson）Perfection V750 Pro扫描仪进行数字化扫描。本图像可用于定量分析。样本制备遵循标准流程：经戊二醛固定、四氧化锇后固定，乙醇梯度脱水，环氧树脂包埋，最终制备得到厚度约90nm的超薄切片。